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- 禾午生物
- P70712
- 国产
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
pCMV-MYC(human)-6×His-Blast
- 保质期：
3个月~12个月
- 供应商：
上海禾午生物有限公司
- 保存条件：
负20℃＆负80℃
- 规格：
干粉＆甘油菌
| 名称 | pCMV-MYC(human)-6×His-Blast |
| 别称 | NM_002467.6;bHLHe39; c-Myc; MRTL; MYCC; |
| 基因长 | 1362bp |
| 质粒宿主 | 哺乳细胞 |
| 质粒用途 | 蛋白表达 |
| 片段类型 | ORF |
| 片段物种 | 人 |
| 原核抗性 | Amp |
| 筛选标记 | Blast |
| 启动子 | CMV |
| 复制子 | pUC |
| 感受态 | DH5a |
| 温度 | 37度 |
| 背景载体 | pCDNA6-Myc-HisB |
| 正向引物 | CMV-F |
| pCMV-FLAG-NPFFR1(human)-Neo |
| pCAG-MAP2K4(human)-3×FLAG-Neo |
| pCMV-EGFP-WDR33(human-Toxic-opt)-Neo |
| pCMV-3×FLAG-LIMK2(human)-Neo |
| pCAG-MAPK8IP2(human)-3×FLAG-Neo |
| pCAG-MAPK8IP1(human)-3×FLAG-Neo |
| pCMV-MYC(human)-6×His-Blast |
| pCMV-NLS-SRSF5(human)-8×His-Neo |
| pCMV-KANSL3(human)-3×HA-Neo |
| pCMV-STAT5A(human)-3×Myc-Neo |
| pCMV-Cnot3(mouse)-Neo |
| pCMV-EYFP-EIF5(human)-Neo |
| pCMV-NFATC1(human)-3×FLAG-Neo |
| pCMV-3×FLAG-IRGM(human)-Neo |
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文献和实验Chromatin Immunoprecipitation Assays for Myc and N-Myc
Myc and N-Myc have widespread impacts on the chromatin state within cells, both in a gene-specific and genome-wide manner. Our laboratory uses functional genomic methods including chromatin immunoprecipitation (ChIP), ChIP-chip
activated. In fact, aberrantly elevated and/or deregulated activity of Myc is associated with the majority of human cancers. Several switchable mouse transgenic models have been developed and provided insights on the role of Myc in maintaining multiple
In this chapter, we discuss in detail two essential methods used to evaluate the interaction of Myc with another protein of interest: co-immunoprecipitation (Co-IP) and in vitro pull-down assays. Co-IP is a method that, by immunoaffinity
技术资料