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- 抗体名:
Purified anti-mouse I-A/I-E
- 抗体英文名:
Purified anti-mouse I-A/I-E
- 浓度:
0.5 mg/mL
- 应用范围:
免疫荧光,流式
- 宿主:
大鼠
- 供应商:
上海善然生物
- 库存:
现货
- 抗原来源:
大鼠
- 保质期:
2-3年左右
- 适应物种:
小鼠
- 标记物:
Purified
- 克隆性:
单克隆
- 保存条件:
2-8度
- 形态:
液体
- 规格:
50ug
Purified anti-mouse I-A/I-E Antibody
M5/114.15.2
Catalog# / Size 107601 / 50 µg
107602 / 500 µg
Clone
Regulatory Status RUO
Other Names MHC class II
Isotype Rat IgG2b, κ
Description These class II molecules are expressed on antigen presenting cells (including B cells) and a
subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells
expressing CD3/TCR and CD4 proteins.
b,d,q,r
Product Details
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen Activated C57BL/6 mouse spleen cells
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography.
Concentration 0.5 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C.
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-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
at 1:200 dilution) Cy3-conjugated affinity-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
cut paraffin sections and deparaffinize with xylene and graded EtOH into PBS. Block slides with 1/20x serum/PBS (use the appropriate blocking serum, i.e. same as 1º Ab), 10'. Wash in PBS 2' x3. Add digoxigenin labeled 1º antiobody.
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