- ￥1511
- BioLegend
- 101335
- 美国
- 2025年07月15日
- 免疫荧光，流式
- 大鼠
- 小鼠
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- 抗体名：
Purified anti-mouse CD16/32 (Maxpar® Ready)
- 抗体英文名：
Purified anti-mouse CD16/32 (Maxpar® Ready)
- 浓度：
1.0 mg/ml
- 应用范围：
免疫荧光，流式
- 宿主：
大鼠
- 供应商：
上海善然生物
- 库存：
现货
- 抗原来源：
大鼠
- 保质期：
2-3年左右
- 适应物种：
小鼠
- 标记物：
Purified
- 克隆性：
单克隆
- 保存条件：
2-8度
- 形态：
液体
- 规格：
100 μg
Purified anti-mouse CD16/32 (Maxpar® Ready) Antibody
93
Catalog# / Size 101335 / 100 µg
Clone
Regulatory Status RUO
Other Names Fcγ R III/II, Ly-17
Isotype Rat IgG2a, λ
Description CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are
expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and
dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate
adaptive immune responses.
Product Details
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen Sorted pre-B cells
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation The antibody was purified by affinity chromatography.
Concentration 1.0 mg/ml
Storage & Handling The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
Application FC - Quality tested
CyTOF® - Verified
Recommended Usage This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using
Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding
100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with
the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar®
Ready antibodies.
Application Notes Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of
immunoglobulins, but is not the same clone as 2.4G2.
The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for
the relevant formats) include: immunoprecipitation and blocking of Fc-mediated reactions in
functional studies . It is useful for blocking non-specific binding of immunoglobulin to Fc
receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with
purified anti-CD16/CD32 antibody (=1.0 µg per 10 cells in 100 µL volume) for 5-10 minutes on ice
prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified
antibody (Cat. No. 101330) (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered).
1
2,4,23
6
Additional Product Notes Maxpar® is a registered trademark of Standard BioTools Inc
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