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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Ng35 Peptide - middle region (AAP31670)
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
100ug
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文献和实验Identification of Anaplastic Lymphoma Kinase Variant Translocations Using 5RACE
of novel chimeric proteins that retain the C-terminal portion of ALK, where the tyrosine kinase domain is located. In most of these tumors, the t(2;5)(p23;q35) translocation causes fusion of the ALK gene to the 5′ region of the nucleophosmin (NPM ) gene
and negative control be conducted in parallel to all analyses. The positive control can be a marker or known protein while the negative control should be a blank region of the gel. Wash twice with 50µL of 18 Mohm water for approx 15 minutes. Wash
that's too complicated. 1) Synthesise 2 complementary oligonucleotides.(see notes) 2) Prepare reaction mixture: 5 ul of 10X reaction buffer X ul of template DNA (5-50 ng) X ul of each primer 1 ul of 10 mM dNTP mix (2.5 mM each dNTP)
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