CheKine™ 细胞壁不溶性酸性转化酶（B-AI）活性检测

CheKine™ 细胞壁不溶性酸性转化酶（B-AI）活性检测试剂盒（微量法）

产品基础信息

货号：KTB2293 | 英文名称：CheKine™ Mirco Cell-Wall Binding Acid Invertase (B-AI) Activity Assay Kit

产品分类：核心产品 | 应用领域：细胞代谢分析 | 样本类型：植物组织

产品背景与原理

蔗糖转化酶（Invertase，Ivr）是高等植物蔗糖代谢关键酶，催化蔗糖不可逆分解为果糖和葡萄糖。细胞壁不溶性酸性转化酶（B-AI）结合于细胞壁，最适pH为3~5，参与韧皮部质外体卸载时蔗糖分解，维持库源间蔗糖浓度梯度。

检测原理：B-AI催化蔗糖降解生成还原糖，与3,5－二硝基水杨酸反应形成棕红色氨基化合物，在510 nm处测定吸光度变化速率，定量B-AI活性。

试剂盒组分

• Extraction Buffer I
• Extraction Buffer Ⅱ
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard

规格与订购信息

操作注意事项

• 推荐使用新鲜样本，-80℃保存样本需控制解冻温度与时间，室温解冻后4 h内完成检测。
• 实验前确保所有组分及设备处于适宜温度。

保存与运输

保存条件：4℃避光保存，有效期6个月 | 运输方式：冰袋运输（蓝冰）

免责声明

警告：本产品仅限研究使用，不适用于人类或临床诊断。应用场景不构成专利或许可证使用建议，对可能产生的侵权或违规行为不承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Cell-Wall Binding Acid Invertase (B-AI) Activity Assay Kit

Basic Product Information

Catalog Number: KTB2293 | English Name: CheKine™ Micro Cell-Wall Binding Acid Invertase (B-AI) Activity Assay Kit

Product Category: Core Products | Application Area: Cell Metabolism Analysis | Sample Type: Plant Tissue

Background and Principle

Invertase (Ivr) is a key enzyme in sucrose metabolism in higher plants, catalyzing the irreversible breakdown of sucrose into fructose and glucose. Cell-Wall Binding Acid Invertase (B-AI) is bound to the cell wall, with an optimal pH of 3~5, and participates in the breakdown of sucrose during phloem apoplastic unloading, maintaining the sucrose concentration gradient between source and sink.

Assay Principle: B-AI catalyzes the degradation of sucrose to produce reducing sugars, which react with 3,5-dinitrosalicylic acid to form a brownish-red amino compound. The rate of absorbance change at 510 nm is measured to quantify B-AI activity.

Kit Components

• Extraction Buffer I
• Extraction Buffer II
• Reagent I
• Reagent II
• Reagent III
• Standard

Specifications and Ordering Information

Operational Precautions

• Fresh samples are recommended. For samples stored at -80°C, control the thawing temperature and time, and complete the assay within 4 hours after thawing at room temperature.
• Ensure all components and equipment are at the appropriate temperature before the experiment.

Storage and Shipping

Storage Conditions: Store at 4°C in the dark, valid for 6 months | Shipping Method: Ice pack shipping (blue ice)

Disclaimer

Warning: This product is for research use only and is not intended for human or clinical diagnosis. The application scenarios do not constitute patent or license use recommendations, and we are not responsible for any potential infringement or violation.

Note: The above information may change due to product optimization and upgrades. For specific information, please consult Yakeyin Company.