Bachem Amyloid β-Protein (3-42

Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy

gradients have been most frequently used, but we prefer 15�40% glycerol gradients in 0.2 M ammonium bicarbonate, because this is the buffer used for rotary shadowing EM (Section   6 ). The protein of interest is sedimented in one bucket of the swinging

【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）

-adrenergic receptorS*, 25 nmol/g, α-MF[161]ADAR1, ADAR2,ds-RNA-specific adenosine deaminasesI, 1 mg/L[200]Alzheimer’s disease amyloid precursor protein α, β, and γ-secretase productsS, PHO1[201]Alzheimer’s disease amyloid precursor protein, 2 domainsS, 24 mg

In Vitro Transcription Assay (Run-off Assay) using Permeabil

that contains DNA and protein. 9. Add 1 volume of 100% Isopropanol (or 2 volumes of 100% Ethanol). 10. Incubate at -20℃ for at least 1 hr to precipitate the RNA. 11. Centrifuge at 10,000 X g for 20 min at 4℃ to pellet the RNA. 12. Transfer the pellet