Baculovirus Quantitative Titra

The Baculovirus Quantitative Titration Kit serves as an extraction and titration system for fresh (less than 3 months old) budded baculovirus stocks based on AcMNPV baculoviral vectors. Ultra-sensitive TaqMan qPCR technique and accurate titrated virus stocks are employed in this kit, providing an alternatively time-saving method of titration up to 2~3 hours. Therefore, reliable multiplicity of infection (MOI) can be determined real-timely, substituting traditional plaque assays or antibody-based assays which usually take 3~4 days.

The primers and probe included in this kit are specific to gp64 that encodes the essential envelope protein GP64, and the sequences, concentration and ration are also optimized. Virus Lysis Buffer from this kit can degrade viral protein, releasing viral DNA that can be used directly as template for qPCR without further purification. Virus Standard, Positive Control (PC) and negative template control (NTC) should be set up every time of quantitative titration.