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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
北京云肽生物科技有限公司
本品用于分离猪胰岛细胞
【产品规格】
2×200ml/Kit
【产品组成】
为方便广大用户使用,试剂内容如下:
|
|
名称 |
产品编号 |
规格 |
|
A |
分离液1 |
|
200ml |
|
B |
分离液2 |
|
200ml |
|
C |
匀浆冲洗液(赠品) |
F2013TBD |
200ml |
|
D |
样本稀释液(赠品) |
2010C1119 |
200ml |
|
E |
清洗液(赠品) |
2010X1118 |
200ml |
|
F |
洗涤液(赠品) |
TBDTM-W |
200ml |
|
G |
说明书 |
|
1份 |
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文献和实验Electroporation of P. aeruginosa
Electroporation of P. aeruginosa Preparation of Electrocompetent Cells Inoculate a 5-ml of L-broth with cells of the P . aeruginosa strain to be electrotransformed. Grow culture overnight at 37°C
BACs, PACs, P1s and Plasmid Isolation Protocol (standard protocol)
with a volume at least 4 times the volume of the culture. 2. Pellet 1.5-5 ml bacteria by centrifugation at 13,000 x g for 3 min at room temperature. Decant or aspirate medium and discard. 3. Resuspend the bacterial pellet by adding 200 ul of Buffer T1/RNase
E.Z.N.A. Endo-Free BACs, PACs, P1s Protocol
with vigorous shaking (~ 300 rpm). Use a flask with a volume at least 4 times the volume of the culture. 2. Pellet 1.5-5 ml bacteria by centrifugation at 13,000 x g for 3 min at room temperature. Decant or aspirate medium and discard. 3. Resuspend
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