分离液 *Sigma货号：P4937-25ML/P4937-

一般描述

Percoll®是一种用于细胞、病毒和亚细胞颗粒密度梯度离心的经典培养基
它由直径为15-30 nm（在水中为23％ w/w）并涂覆有聚乙烯吡luò烷酮（PVP）的胶体二氧化硅颗粒组成
PVP涂层使产品完全无毒，非常适合与生物材料一起使用
由于其颗粒大小的不均匀性，沉积以不同的速率发生，自发地在1.0-1.3 g/ml范围内产生非常光滑的等距梯度
应用
Percoll®已用于以下材料的离心分离：
新生大鼠心室心肌细胞[1]
血液样本中的白细胞[2]
小管周围肌样（PM）细胞[3]
适用于制备单细胞悬浮液以进行测序
Percoll is composed of silica gel particles with a particle size of 15-30nm (23% w/w in water), which are coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic, low viscosity, and low osmotic pressure density gradients, which can be used for separating cells, organelles, and viruses. It has been used to separate the inner and outer membrane vesicles of Escherichia coli. Percoll is isotonic, non-toxic, and easily removed from purified materials throughout the entire gradient process. Characteristics and advantages Low osmotic pressure allows for precise adjustment of physiological conditions without significant interference from the medium. Compatible with live cells and viruses, and allows for the isolation and recovery of complete, fully active systems. It is impermeable to biofilms and does not change the buoyancy density of particles during centrifugation. The gradient spontaneously formed during centrifugation allows for the mixing of a large amount of samples in the centrifuge tube. Low viscosity leads to rapid gradient formation and particle separation. Most biological particles with a settling coefficient greater than 60S can be successfully separated in Percoll gradient