Polybrene-Plus

Polybrene/DMSOAssisted Gene Transfer

, several laboratories have turned to the use of synthetic polycations for delivering copious amounts of exogenous DNA to target cells without compromising their viability or clonogenicity. One of these compounds, polybrene, is mostly known for its ability to enhance

DNA Transfection of Mammalian Cells Using Polybrene

of the polycation polybrene, followed by a DMSO shock. The polybrene apparently interacts with the charge on the cell and the DNA, allowing the DNA to absorb more easily to the cell membrane.

凝聚胺（polybrene）的使用体会

  在很多医院输血科和血站血型室，凝聚胺已被广泛用于抗体筛查和交叉配血，现在就我们对凝聚胺的使用谈谈体会，一家之言，希望大家批评。 1 LIM的用量：大部分市售凝聚胺试剂盒使用说明书建议LIM 0.6ml；我们建议LIM 1.0ml.。 2 2滴血清+1滴2-4%红细胞后加入LIM混匀后，建议：RT放置1分钟，这样有利于弱抗原抗体结合（抗原抗体反应的第一阶段）。 3 加入2滴凝聚胺混匀后，建议：15秒后离心，这样有利于致敏红细胞联结（抗原抗体反应的第二阶段）。