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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] *10 mM in ddH2O*
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
1umole
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文献和实验Single Primer ("Semi-Random") P
Reamplification 40 µL H2O 10 µL 10x M-PCRB 10 µL 4 dNTPs @ 2 mM each 10 µL Pn primer @ 5 µM 10 µL Po primer @ 0.2 µM 10 µL DNA from part 1 10 µL "T/TS" @ 0.4 u Taq/µL --------- 100 µL Load glass capillaries and amplify: 30"x94°C 30
Preparation of Poly A+ RNA and Northern Analysis
one on the long 3 MM piece and wet with 10 x SSC, (vi) a 6-8 inch stack of paper towels cut to the same size as the gel, (vii) a glass tray containing ddH2O, (viii) a glass tray containing 10 x SSC, (ix) two 10Ó x 12Ó pieces of X-ray film, and (x) flat forceps
is carried out exactly as in Northerns.Transfer gel to a clean glass tray and immediately denatured in 900 ml of 50 mM NaOH/10 mM NaCl (to 893.7 ml of ddH2O add 4.5ml of 10 N NaOH and 1.8ml of 5 M NaCl) for 20min on a rotator.Carefully pour off and add 900 ml
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