Concanavalin A, XFD488 Labeled

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cells treated with 1 µM camptothecin. The externalized phosphatidylserine, a characteristic of early-stage apoptotic cells, was detected with Alexa Fluor 488 annexin V (A13201). The late-stage apoptotic and necrotic cells were stained with propidium

Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded Ketone Functionalities

to the amber stop codon TAG. Proteins were purified with high yield and subsequently labeled with the hydroxylamine derivative of Alexa Fluor� 488 functioning as a fluorescent donor dye. The proteins were then labeled via maleimide coupling chemistry

Atomic Force Microscopy for Analyzing Metaphase Chromosomes: Comparison of AFM Images with Fluorescence Labeling Images of Banding Patterns

with 5-ethynyl-2′-deoxyuridine (EdU) during the late S phase. EdU-labeled DNA is detected in metaphase chromosomes using Alexa Fluor 488� azide, through the 1,3-dipolar cycloaddition reaction of organic azides with the terminal acetylene group of EdU. Chromosomes