EliKine™小鼠颗粒酶B ELISA定量试剂盒

EliKine™小鼠颗粒酶B ELISA定量试剂盒（KTE3018）

产品概述

应用领域：ELISA法检测小鼠样本中颗粒酶B浓度（细胞培养上清/血清/血浆）

检测原理：双抗体夹心法，通过特异性抗体捕获目标蛋白，结合生物素化检测抗体与链霉亲和素-HRP显色系统实现定量分析。

技术参数

• 检测范围：0.31 ng/mL - 20 ng/mL
• 灵敏度：0.31 ng/mL
• 样本类型：细胞培养上清｜血清｜血浆
• 种属反应性：小鼠特异性

试剂盒组分

• 预包被微孔板
• 冻干标准品
• 10×检测缓冲液
• 生物素化检测抗体
• 链霉亲和素-HRP复合物
• TMB显色底物
• 终止液

核心优势

• 高特异性：无交叉反应干扰
• 超敏检测：可识别0.31 ng/mL微量蛋白
• 稳定可靠：板内板间变异系数＜10%
• 便捷操作：4小时内完成完整检测流程

实验方案

规格	价格	货期
48 T	¥1598	期货（4天内发货）
96 T	¥2558	期货（4天内发货）

注意事项

• 不同批次试剂禁止混用
• 需严格进行复孔检测
• 使用前需室温平衡30分钟
• 推荐使用低速振荡器混匀

保存与运输

• 保存：2-8℃避光，开封后按组分要求保存
• 运输：蓝冰低温运输

警告：本产品仅供科研使用，不适用于临床诊断。使用前请充分阅读产品说明书。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Mouse Granzyme B ELISA Quantitation Kit (KTE3018)

Product Overview

Application: Quantitative detection of mouse Granzyme B in biological samples (cell culture supernatant/serum/plasma) by ELISA

Principle: Sandwich ELISA format utilizing specific capture antibodies, biotinylated detection antibodies, and streptavidin-HRP chromogenic system for quantitative analysis.

Technical Specifications

• Detection Range: 0.31 ng/mL - 20 ng/mL
• Sensitivity: 0.31 ng/mL
• Sample Types: Cell culture supernatant | Serum | Plasma
• Species Reactivity: Mouse specific

Kit Components

• Pre-coated microplate
• Lyophilized standard
• 10× Assay Diluent
• Biotinylated detection antibody
• Streptavidin-HRP conjugate
• TMB substrate solution
• Stop solution

Key Features

• High Specificity: No cross-reactivity observed
• Ultra-Sensitive Detection: Detects as low as 0.31 ng/mL
• Reproducible Performance: Intra- and inter-assay CV <10%
• Rapid Protocol: Complete analysis within 4 hours

Ordering Information

Format	Price	Delivery
48 T	¥1598	In stock (shipped within 4 days)
96 T	¥2558	In stock (shipped within 4 days)

Precautionary Notes

• Do not mix reagents from different batches
• Duplicate measurements are required
• Equilibrate to room temperature for 30 minutes before use
• Low-speed orbital shaker recommended for mixing

Storage & Shipping

• Storage: 2-8°C protected from light. Store components separately after opening.
• Transport: Shipped with blue ice for low-temperature transport

Warning: For research use only. Not for diagnostic procedures. Read the manual thoroughly before use.

Note: Specifications are subject to change due to product optimization. Contact Yakonin Biotech for latest information.