- ￥5340
- 华韵生物(Huayunbio)
- HYPC5203
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
广州华韵生物科技有限公司
- 规格：
10µg
产品信息
| 别名 | Interleukin-5; IL-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF; IL5 |
| 物种 | Human |
| 表达宿主 | Human Cells |
| 序列信息 | Ile20-Ser134 |
| 检索号 | P05113 |
| 分子量 | 14.2 kDa |
| 表观分子量 | 20 kDa |
| 标签 | C-6His |
| 生物活性 | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.The ED50 for this effect is 0.1-0.5 ng/ml. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2~8℃ for 2~7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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文献和实验ELISPOT (Enzyme-linked ImmunoSPOT)
for detection of antigen-specifi c memory effector CD4 and CD8 cell populations in human diseases. J. Immunol. Methods. 285: 223-235. Smith JG, et al. 2001. Development and validation of a gamma interferon ELISPOT assay for quantitation of cellular immune
virus solution at a density of 1-4 x 104 pfu/ml (see Quick Guide Chart for human IFN-gamma: 1/250 dilution of stock of 107 pfu/ml, for mouse IFN-gamma, 1/1000 dilution of stock of 107 pfu/ml). Incubate plate for 40 hours at 37°C, 5% CO2
网络 二、免疫细胞中的凋谢 各种免疫细胞都存在有凋谢的现象，这对保持免疫系统的平衡十分重要。诱导免疫系统PCD的因素有生理性和非生理性的。生理性因素如细胞因子浓度的改变、B细胞发育过程中Ig基因的重排等。非生理地因素如电离辐射、加热、药物等。体外培养的淋巴细胞对电离辐射尤为敏感，即使暴露在2-5Rad下也能产生形态改变，X身线和Y射线可诱导静止期的淋巴
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