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huayun / IL-38/ IL-1F10, Human

, Recombinant,HYPC5199
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  • ¥5340
  • 华韵生物(Huayunbio)
  • HYPC5199
  • 2025年07月13日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      广州华韵生物科技有限公司

    • 规格

      10µg

    产品信息

    别名Interleukin-1 Family Member 10; IL-1F10; FIL1 Theta; Interleukin-1 HY2; IL-1HY2; Interleukin-1 Theta; IL-1 Theta; IL1F10; FIL1T; IL1HY2; IL-38
    物种Human
    表达宿主E.coli
    序列信息Met1-Trp152
    检索号AAI03967.1
    分子量16.9 kDa
    表观分子量17 kDa

    产品特性

    纯度 >95% as determined by reducing SDS-PAGE.
    内毒素<1.0 EU per µg as determined by LAL test.
    保存 Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2~8℃ for 2~7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months.
    运输 Ambient temperature or ice pack.
    制剂Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 6.0.
    复融Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

    背景介绍

    Human Interleukin 1 Family Member 10 (IL-1F10) is thought to participate in a network of Interleukin 1 Cytokine family members to regulate adapted and innate immune responses. IL-1F10 was expressed in fetal skin, spleen and tonsil, mostly in the basal epithelia of skin and in proliferating B-cells of the tonsil. IL-1F10 binds soluble IL-1 Receptor type 1 and may be implicated in regulating adapted and innate immune responses. Two alternatively spliced transcript variants encoding the same Protein have been reported.

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    图标文献和实验
    相关实验
    • L929细胞增殖MTT比色法检测IL-1的生物活性

      仪以检测波长570nm,参考波长630nm,分别测定各孔OD值。测定应在酸化异丙醇加入后lh内完成。 【结果分析】 (1)     每孔OD值应为OD570 nm-OD630 nm,再减去培养液对照OD值,最终每孔OD值应取3复孔的平均值; (2)     以log2 [稀释度]为X(横座标),各稀释度对应的OD值为Y(纵座标),在普通坐标纸上,分别绘制出IL-1标准品与待测样品两条回归曲线(如图)。   IL-1标准品与待测样品两条回归

    • Activation and Expansion of Human Treg Cells

      一、实验原理 Dynabeads Human Treg Expander offers a simple method for expansion of Treg cells that does not require antigen- presenting cells or antigen. Just add Dynabeads? Human Treg Expander and recombinant IL-2 (rIL-2) to the Treg culture

    • Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood

      of exogenous genes. Infected cells are cultured in hematopoietic medium (X-VIVO 10 containing 50 ng/mL IL-6, 50 ng/mL soluble IL-6 R, 50 ng/mL SCF, 10 ng/mL TPO, and 20 ng/mL Flt-3-ligand) for 5 days after viral infection. Then, cells are transferred onto SNL76

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