- ￥4140
- 华韵生物(Huayunbio)
- HYPC5090
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
广州华韵生物科技有限公司
- 规格：
10µg
产品信息
| 别名 | Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; LIF; HILDA |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | Ser23-Phe202 |
| 检索号 | P15018 |
| 分子量 | 19.7 kDa |
| 表观分子量 | 18 kDa |
| 生物活性 | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 25-150 pg/ml. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2~8℃ for 2~7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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文献和实验. N 6-methyladenosine modification-mediated mRNA metabolism is essential for human pancreatic lineage specification and islet organogenesis. Nature Communications, 2022, 13.1: 4148. 【5】Zhu S, Russ H A, Wang X, et al. Human pancreatic beta-like cells
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
. 36. Resuspend the cells in OP9 medium, seed 5 x 105 cells per 10-cm dish containing ~80% confluent OP9 cells in 10 mL of OP9 medium, and add 5 ng/mL of recombinant human Flt-3L. Return the dish to the incubator. The number of 10-cm dishes
At least 6 x 106 cells are needed to proceed with the transfection. 8. Plate 3 x 106 mES cells in 10 mL of mES cell growth medium with LIF into a gelatin-coated, 10-cm tissue culture dish. Prepare two 10-cm dishes per transfection.
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