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- 华韵生物(Huayunbio)
- HYPC4758
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- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
广州华韵生物科技有限公司
- 英文名：
详见说明书
- 规格：
500mL
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文献和实验Enrichment of PBMCs with monocytes
for FACS analysis and proceed with MACS separation. Continuous Percoll gradient preparation: * : 13.5 ml Percoll (Amerhsam), 15.0 ml MEM Spinner modification (Sigma), 1.5 ml 10x Earle’s salts solution (Biochrom) Centrifuge 10 min
Monocytes/Macrophages: Enrichment of PBMCs with Monocytes
preparation: Mix in 50 ml tube*: 13.5 ml Percoll (Amerhsam), 15.0 ml MEM Spinner modification (Sigma), 1.5 ml 10x Earle’s salts solution (Biochrom) Centrifuge 10 min at 14000 x g without breaks in a fixed angle rotor. Carefully
Construction and Characterization of Adenovirus Vectors
as described above or in suspension. Grow the cells in suspension in 1X maintenance medium in suspension flasks, agitated at 70 rpm. When they reach a density of 5 x 105 cells/mL, dilute 1:2 or 1:3 with 1X maintenance medium. CsCl (cesium chloride
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