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- Merck
- SCC490
- 2026年01月22日
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细胞名称：Mouse OP9-DL4-7FS Notch Ligand Cell Line
货号：SCC490
规格：vial of ≥1X10⁶ cells/vial vial
应用：Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.
Cells are verified to be of mouse origin and negative for interspecies contamination from human, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
Cells are negative for mycoplasma contamination.
特点和优势：OP9-DL4-7FS is a stable bone marrow derived stromal cell line expressing the Notch ligand-Delta like 4 (DL4) and human IL-7, FLT3L, and SCF (7FS) for stem cell differentiation applications.
储存及稳定性：OP9-DL4-7FS cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.
其他说明：This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation.
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
with the support of OP9-DL1 cells, a bone-marrow-derived stromal cell line that ectopically expresses the Notch ligand, Delta-like 1 (Dll1). This approach provides a simple, versatile, and efficient culture system that allows for the commitment, differentiation
In Vitro Human T Cell Development Directed by NotchLigand Interactions
cord blood (CB)- derived hematopoietic stem cells (HSCs) using a bone marrow stromal cell line. This protocol is broken into three major steps: (1) the maintenance of a working stock of OP9 bone marrow stromal cells expressing the Notch receptor ligand
Directed Differentiation of Embryonic Stem Cells to the T-Lymphocyte Lineage
cells and B lymphocytes, while the expression of an appropriate Notch ligand, such as Delta-like 1, on OP-9 cells (OP9-DL1) is required to support the generation of T-cells in vitro. Here, we describe an updated and streamlined protocol
