Anti-14-3-3 eta/YWHAH antibody

Introduction of human 14-3-3 proteins

of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins.A polyclonal antibody was raised against purified ST-IV.A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti

类器官培养与应用——血管类器官

A, Aichinger M, et al. Generation of blood vessel organoids from human pluripotent stem cells[J]. Nature protocols, 2019, 14(11): 3082-3100. 【4】Sommer C J. Ischemic stroke: experimental models and reality[J]. Acta neuropathologica, 2017, 133(2): 245-261. 【5】Lu

类器官培养与应用——肾类器官

, 1% BSA 和  0.035% NaHCO3），进行流式分选细胞。 11、分选出的阳性细胞（约 5,000 个）种在 V 型底低吸附的 96 孔板中，210 × g 离心 4 min。 12、将细胞用 Step5 培养基换液，培养 54h。 13、将细胞转移至含有 Step6 的培养孔中，培养 48h。 14、将细胞转移至含有 Step7 的培养孔中，培养 72h，形成 UB 球体。 诱导输尿管芽（Ureteric bud, UB）分枝形成 15、将 UB 球体转移至 24 孔 Transwell 中