Anti-Retinoid X Receptor alpha

Preparation of Polyclonal Antibodies to Retinoid Receptors

of a retinoid “X” receptor α (RXRα) antibody on Western blots contributed to the identification of the RXR ligand by detection of the receptor in fractions of whole-cell lysates that were able to bind the 9-cis retinoic acid ligand (2 ) Antibodies

Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas

by feeding the others (and collecting supernatants for testing) - again remember that most hybridomas will be ADHERENT, so will need vigorous pipetting to get them into suspension for splitting. h) Split wells into 25ml flasks to make 2 x 2 freezes

Immunofluorescence: Rabbit Anti-Murine RELMα

for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide