Anti-Polyoma virus, Large T an

T-cell dependent antigen specific in situ staining (Pe)

if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe

Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction

10 µg/ml anti‐β2µ‐globulin antibody in 10 mM sodium acetate, pH 5.5 1 M ethanolamine 50 mM NaOH

Large-scale immunofluorescence

) or orbital platform shaker (240 r.p.m.). Centrifuge microtiter dishes for 5 minutes at 500 X g to pellet cells. Fix cells by adding 1/10 volume of 37% formaldehyde and incubating at room temperature for 15 minutes to 1 hour 2 without shaking