- ￥1800
- 华韵生物(Huayunbio)
- HY-ab118572-50ul
- 2025年07月13日
- WB,IHC,IF/ICC
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
-20℃
- 适应物种：
Human, Mouse, Rat
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB,IHC,IF/ICC
- 浓度：
~1mg/ml
- 抗体名：
Anti-Ezrin / Radixin / Moesin antibody ，应用: WB,IHC,IF/ICC，反应: H, M, R，HY- ab118572 -50ul
- 规格：
50ul
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
by feeding the others (and collecting supernatants for testing) - again remember that most hybridomas will be ADHERENT, so will need vigorous pipetting to get them into suspension for splitting. h) Split wells into 25ml flasks to make 2 x 2 freezes
Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
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