Biotin Anti-alpha smooth muscl

Isolation of Non-muscle Actin

for isolation of Erythrocyte Actin 1. Collect blood from animal using 20 ml/100 ml whole blood acid citrate/dextrose (0.085 M sodium citrate, 0.065 M citric acid 2% dextrose) as anti-coagulate. 2. Centrifuge blood at for 10 min at 4300 x g and remove

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands

类器官培养与应用——血管类器官

of development of diabetes[J]. Biorheology, 1992, 29(5-6): 443-457. 【7】Basatemur G L, Jørgensen H F, Clarke M C H, et al. Vascular smooth muscle cells in atherosclerosis[J]. Nature reviews cardiology, 2019, 16(12): 727-744. 【8】Zhang Y, Wang S, Dudley A C. Models