- ￥884
- 华韵生物(Huayunbio)
- HFC1862-100μg
- 2025年07月14日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100μg
请联系华韵客服获取说明书。
产品简介：
| Background |
The Cy34.1 monoclonal antibody specifically binds to the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g., A, BALB/c, CBA, C3H/He, C57BL, C57L, C58, SJL, SWR, but not AKR, DBA/1, DBA/2, NZB, PL). CD22 is expressed at high levels on mature peripheral B lymphocytes (follicular and marginal zone), B-1 cells (CD5+ B cells), and plasma cells. It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. Its intracellular domain is phosphorylated after cross-linking of antigen receptor or MHC class II antigen. It is involved in negative regulation of B-cell activation and protection from autoimmunity. B-cell proliferative responses to LPS or anti-mouse Ig µ chain are augmented in the presence of Cy34.1 mAb.
|
| Alternate Names |
Siglec2;B-cell receptor CD22;B-lymphocyte cell adhesion molecule;BL-CAM;CD22;Cd22;Lyb-8;Sialic acid-binding Ig-like lectin 2;Siglec-2;T-cell surface antigen Leu-14
|
| Swissprot |
P35329
|
| Gene ID |
12483
|
| Clone No |
Cy34.1
|
| Host |
Mouse
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
Biotin Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
Biotin
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Dual- and Triple-Color Fluorospot
anti-IFN-γ-FITC (1 μg/ml), mAb anti-IL-2-biotin (2 μg/ml), mAb anti-FITC-green (1 μg/ml), and SA-Cy3 (0.5 μg/ml).
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
免疫组化技术-Immunohistochemical Protocols
in the same section. You can use the above protocols and do the two stainings simultaneously. (A brief example: first antibody (raised in rabbit); anti-rabbit-IgG-FITC; second primary antibody (raised in mouse); anti-mouse-IgG-CY3. Result: one antigen shows
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