- ￥3042
- 华韵生物(Huayunbio)
- HFC1714-100Tests
- 2025年07月16日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
|
| Alternate Names |
CD3E;CD3e;T-cell surface antigen T3/Leu-4 epsilon chain;T-cell surface glycoprotein CD3 epsilon chain;T3E
|
| Swissprot |
P07766
|
| Gene ID |
916
|
| Clone No |
OKT-3
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG2a, κ
|
| Isotype Control |
PE/Cyanine5 Mouse IgG2a, κ Isotype Control[C1.18.4]
|
| Conjugation |
PE/Cyanine 5
|
| Conjugation Information |
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验上 Strep-tag 的 Fab 片段（Fab-Streps）特异性的结合到琼脂糖基质上。随后，加入全血或其他血液制剂流过柱子。目的细胞通过特异性的 Fab-Strep 作用从而结合到琼脂糖基质上，其他非目的细胞则有效的被洗去。最后，加入生物素使目的细胞从 Fab-Streps 上解离。至此，就已分离得到不带任何标记的、真实的目的细胞可用于下游的实验研究。 1. 实验流程 所需产品： CD3 Fab-TACS® Gravity Kit, human（IBA Lifesciences, Cat
45-PerCP PCP-30039 CD3-FITC FITC-30004 CD16-PE PE-30061 CD56-PE PE-30008 CD19-APC APC-30066 Human Th1/Th2 细胞群检测 CD3-PerCP-Cy5.5 PCP55-30004 CD4-FITC FITC-30005 IFN-γ-PE PE-30053 IL4-APC APC-30043
Cell Surface Immunofluorescence Staining Protocol
as the antibodies used for immunofluorescent staining. 4. Cell-Surface Staining with Antibody: Add appropriately conjugated fluorescent, biotinylated, or purified primary antibodies at predetermined optimum concentrations (e.g. anti-CD3-FITC, anti
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