- ￥975
- 华韵生物(Huayunbio)
- HFC1331-100μg
- 2025年07月14日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100μg
请联系华韵客服获取说明书。
产品简介：
| Clone No |
MOPC-21
|
| Host |
Mouse
|
| Application |
FCM
|
| Isotype |
Mouse IgG1, κ
|
| Conjugation |
FITC
|
| Conjugation Information |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
Determination of class and subclass of monoclonal antibodies (MAbs) is important because several immunochemical and functional properties of antibodies depend on the isotype. Knowledge of the light-chain isotype (κ for 95% or λ
择同型对照？ (1)一般选择与一抗的成分完全相同种属来源、相同亚型和亚链、相同荧光标记的抗体，比如抗人的CD56 FITC标记的抗体，成份是MouseIgG1,κ。那么它的同型对照应该是FITC标记的MouseIgG1,κ。注意同型对照的成份跟它的名称是相同的. (2)如果是抗体的组合形式是纯化的一抗＋荧光标记的二抗，那么应该选择一抗的同型对照。比如CD86的纯化抗体，成分是MouseIgG1,κ。它的同型对照是纯化的Mouse IgG1,κ。它的二抗PE标记的抗小鼠IgG1
29 (MEM 101A, purified IgG1) was used as reported previously [9–11]. FITC-labeled antibodies to CD80, CD86, CD29, and CD18 were obtained from Immunotech (Marseilles, France).2.2. AnimalsC57BL/6 male mice (6–8 weeks old, 17–21 g) were obtained from DAEHAN
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