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- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
100μg
请联系华韵客服获取说明书。
产品简介:
| Background |
CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation.
|
| Alternate Names |
T3; and ζ chains; γ; ε;CD3;CD3 Complex;T-cell surface glycoprotein CD3 δ
|
| Swissprot |
P19377;Q64159;D4A5M2;
|
| Gene ID |
25710,300678,315609,25300
|
| Clone No |
G4.18
|
| Host |
Mouse
|
| Reactivity |
Rat
|
| Application |
FCM
|
| Isotype |
Mouse IgG3, κ
|
| Isotype Control |
PE/Elab Fluor® 594 Mouse IgG3, κ Isotype Control[A112-3]
|
| Conjugation |
PE/Elab Fluor594
|
| Conjugation Information |
PE/Elab Fluor 594 is designed to be excited by the blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 620 nm (e.g., a 610/20 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Cruz, CA), anti-CD49f (clone GoH3, PharMingen) and secondary antibodies conjugated to phycoerythrin (PE, 1:100 dilution, Jackson ImmunoResearch, West Grove, PA). Next, 5 105 to 106 GFP+ PE+ infected cells (3ZF library) or 107 GFP+ PE+ infected cells
secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)Secondary Antibody250µlIF-IC HCA4414SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4416SAnti-rat IgG (H+L), (Alexa Fluor® 488 Conjugate)Secondary
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