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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
100Tests
请联系华韵客服获取说明书。
产品简介:
| Background |
Interleukin 21 (IL-21) is a potent immunomodulatory cytokine mainly produced by NKT and CD4+ T-cells, particularly the inflammatory Th17 subset, and has pleiotropic effects on both innate and adaptive immune responses. These actions include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells. It can also be proapoptotic for B cells and NK cells. Studies have shown that IL-21 is also an autocrine cytokine that potently induces Th17 differentiation, suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.
|
| Alternate Names |
IL-21;Interleukin-21;Za11
|
| Swissprot |
Q9HBE4
|
| Gene ID |
59067
|
| Clone No |
3A3-N2
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
ICFCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
PE Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
避光染色30分钟至过夜。 最后用相同破膜缓冲液洗涤两次,重悬后通过流式细胞仪采集数据。该方案兼顾报告荧光稳定性与核/胞质蛋白染色效率,适用于多参数精细免疫表型分析。 菲恩生物提供专业流式配色设计与高质检测试剂,优化多色方案,降低干扰,提升检测精准度,助力免疫细胞精细分选,赋能免疫学、肿瘤免疫及自身免疫疾病研究。欢迎合作,共促创新! 以下是菲恩生物推荐的流式抗体搭配方案 种属 细胞群 流式抗体搭配 货号 Human T/B/NK细胞群检测 CD
Intracellular Immunofluorescent Staining for Flow Cytometry
Monensin eBio64CAP17 , eBio64DEC17 IL-21 PBMC PMA (30-50ng/ml)/Iono (1ug/ml) 4-7hr or 12-18hr Brefeldin A eBio3A3-N2 MCP
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