- ￥585
- 华韵生物(Huayunbio)
- HFC2561-25μg
- 2025年07月12日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
25μg
请联系华韵客服获取说明书。
产品简介：
| Background |
IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
|
| Alternate Names |
IFN-gamma;IFNγ;Ifng;Interferon gamma
|
| Swissprot |
P01580
|
| Gene ID |
15978
|
| Clone No |
XMG1.2
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
ICFCM
|
| Isotype |
Rat IgG1, κ
|
| Isotype Control |
FITC Rat IgG1, κ Isotype Control[HRPN]
|
| Conjugation |
FITC
|
| Conjugation Information |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Dual- and Triple-Color Fluorospot
anti-IFN-γ-FITC (1 μg/ml), mAb anti-IL-2-biotin (2 μg/ml), mAb anti-FITC-green (1 μg/ml), and SA-Cy3 (0.5 μg/ml).
Intracellular Immunofluorescent Staining for Flow Cytometry
mouse spleen ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized) + anti-CD28 (2ug/ml soluble) 5hr Brefeldin A XMG1.2 IL-1α mouse
注：此后步骤应注意避光操作。 1.2 滴加一抗 将4 μl Anti-DIG monoclonal antibody 和0.5 μl Texas-red-Avidin 稀释于100 μl 抗体稀释液1中，混匀；每张玻片滴加25 μl 于杂交区域，盖上22mm×22mm盖玻片，于37℃湿盒中孵育1小时；室温下4×SSC/0.1 % Tween-20中振荡洗涤5min×2缸。 1.3 滴加二抗
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