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文献和实验with 250 µl Pierce Coomassie protein assay reagent in 96-well ELISA dishes and reading at 650 nm on a plate reader).The foregoing affinity purification steps were carried out at 4ºC.The proteins were dialyzed against PBS and stored at –20ºC.Protein
Antigen Design Sera Purification Tech Sheet
to recognize that the immune system reacts to the peptide-protein carrier as a whole and that there will be a portion of response directed against the conjugated peptide as well as the linker and the carrier protein1. When screening by ELISA it is advisable
Antigen Design Sera Purification Tech Sheet
directed against the conjugated peptide as well as the linker and the carrier protein1 . When screening by ELISA it is advisable to use a peptide conjugate prepared using a different carrier protein. This is not necessary if performing ELISA assays
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