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文献和实验Transcriptional Regulation of Genes via Hypoxia-Inducible Factor
Hypoxia-inducible factor (HIF) is the principal transcription factor that regulates adaptive physiologic responses to compromised oxygen tension. von Hippel–Lindau (VHL) tumor-suppressor protein binds and ubiquitylates the catalytic α subunit
Hypoxia-Inducible Factors: Post-translational Crosstalk of Signaling Pathways
Hypoxia-inducible factor-1 (HIF-1) has a central role in the mammalian program by which cells respond to hypoxia in both physiological and pathological situations. HIF-1 transcriptional activity, protein stabilization, protein–protein
DNA-Binding Activity of Hypoxia-Inducible Factors (HIFs)
in a supershift, individual proteins or protein complexes in DNA-binding activities can be identified. There is no single protocol that works equally for all proteins. We describe here a protocol designed to investigate DNA-binding properties of hypoxia-inducible
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