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文献和实验their signal by binding to a fibroblast growth factor receptor (FGFR) which has an intracellular protein tyrosine kinase domain. The tyrosine kinase domain is activated upon FGF binding, resulting in the activation of a transcription factor by means of a signal
Ultrasensitive ELISA for Measurement of Human Cytokine Responses in Primary Culture
ELISAs offer excellent specificity and, once fully optimized, sensitivity that rivals that of bioassays. The major variables that need to be experimentally determined when developing an ELISA are the optimal number of fresh cells required
Maintenance of Chicken Embryonic Stem Cells In Vitro
In this chapter, we describe the methods we have used to show that chicken leukemia inhibitory factor (LIF) maintains chicken embryonic stem (ES) cells in an undifferentiated state in culture. Recombinant chicken LIF (rchLIF) was expressed
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