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文献和实验Expression of Neurotrophic Factors such as GDNF in the E. coli System
Neurotrophic factors play an essential role in the growth, survival, and differentiation of neurons in the nervous system. Several well-characterized neurotrophic factors, such as nerve growth factor (NGF), brain-derived
Identification of Neurotrophic Factors by Molecular Biological Techniques
Many proteins have “neurotrophic” activities in in vitro or in vivo test systems. Originally these were discovered as certain neurotrophic activities with unknown molecular structures. Nerve growth factor (NGF), brain derived neurotrophic
Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain
with an antibody. Here we describe materials and methods used to perform basic indirect immunohistochemistry and in situ hybridization on frozen sections through the developing chicken brain, emphasizing controls and potential problems that may be encountered.
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