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文献和实验Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice
the expression of mouse heavy or light chains, the endogenous mouse Ig (mIg) loci must be silenced by gene-targeting techniques. Subsequently, to obtain antigen-specific mAbs, conventional immunization protocols can be followed and the mAb technique used (fusion
Generation and Cloning of Antigen-Specific Human T-Cells
in the presence of TCGF-containing supernatant only, long-term cultures of cloned human allo-antigen-specific T-cell lines (3 , 4 ) needed repetitive stimulation with specific allo-antigen for then growth (3 ) This led to the general assumption that antigen
Antigen-Specific In Vitro Immunization: A Source for Human Monoclonal Antibodies
Human monoclonal antibody has great potential for treatment of various diseases utilizing their specificity against antigens. We have shown an in vitro immunization (IVI) protocol inducing antigen-specific immune responses in human peripheral
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