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Human CREB (Cyclic AMP Response Element Binding Protein) ELISA Kit实验中的注意事项:1.切记在加酶试剂后用吸水纸在酶标板表面轻拭吸干Human CREB (Cyclic AMP Response Element Binding Protein) ELISA Kit2.合理安排检测量,以免反应板过多造成洗板等待时间长。Human CREB (Cyclic AMP Response Element Binding Protein) ELISA Kit3.在吸取液体时,要用量程和需要量接近的枪去吸,减少误差。Human CREB (Cyclic AMP Response Element Binding Protein) ELISA Kit4.务必做双孔实验,这样才既能保证数据的准确性,又能反映出试剂盒的精密度。
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发表【中文论文】请标注:由上海酶研生物科技有限公司提供;发表【英文论文】请标注:From Shanghai EK-Bioscience Biotechnology Co., Ltd.
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policeman.4. Put the extract into an eppendorf and boil for 5 min., cool on ice.5. Load onto 10% SDS-PAGE. I usually load about 1/5 of the lysate from a 60 mm dish. This is about 100 μg protein for PC12 cells.6. After electrophoresis, electrotransfer
-responsive-element binding protein, in the regulation of neuronal plasticity and memory formation.
mm dish. This is about 100 ug protein for PC12 cells. After electrophoresis, electrotransfer to nitrocellulose membrane. I include in the SDS-PAGE prestained molecular weight markers to check the efficiency of the transfer
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