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文献和实验Affinity Purification of Proteins Binding to GST Fusion Proteins
, or maltose?binding protein fusions (MBP), and these may offer particular advantages. A describes preparation of an E. coli extract that is added to the reaction mixture with purified test protein to reduce nonspecific binding.
.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
pRB SBF (Swi4, Swi6) E2F-1, E2F-2 MBF (Mbp1, Swi6)
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