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文献和实验our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
Solid-Phase Assays of Receptor-Binding Specificity
similar to standard ELISA. Each assay determines binding of the virus immobilized in the wells of 96-well plate to receptor analogues in solution. In the direct binding assay, the virus binds to either synthetic biotinylated sialylglycopolymers
Protein Carbonyl Measurement by ELISA
Protein carbonyls are formed by a variety of oxidative mechanisms and are sensitive indices of oxidative injury (1). The conventional assay for protein carbonyls is a colorimetric procedure that measures binding of dinitrophenylhydrazine (DNP
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