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文献和实验Protein A Purification of Antibody
fractions until base line is achieved.Elute Ab with 5 bed volumes of Elution Buffer. Collect 1 ml fractions into 50 ml 1M Tris, until base line is achieved.Take 50 ml aliquots of each fraction for ELISA.Pool fractions containing Ab. Dialyze against 2 x 4L
Production of Bispecif ic and Trispecific F(ab)2 and F(ab)3 Antibody Derivatives
of two protein surfaces, for example, antigen and detecting agent in lmmunohis-tochemistry or immunoassays. More interestingly, BsAbs have been shown to have considerable therapeutic potential when used as targeting reagents to deliver pharmacological substances
Protein Carbonyl Measurement by ELISA
) (2,3). Protein-bound DNP can also be measured with increased sensitivity by enzyme-linked immunosorbent assay (ELISA) (4,5). Samples containing protein are reacted with DNP, then the protein is nonspecifically adsorbed to an ELISA plate. Unconjugated DNP
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