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文献和实验Rac and Rap GTPase Activation Assays
The detection of Ras superfamily GTPase activity in neutrophils is important when studying signaling events elicited by various ligands and cellular processes. Substantial progress in monitoring GTPase activation has been made in recent years
上释放出来,取而代之的是GTP,从而将Ras激活,GEF的活性受生长因子及其受体的影响。另一个控制Ras蛋白活性的是GTP酶激活蛋白(GTPase activating protein, GAP),存在于正常细胞中,主要作用是激活Ras蛋白的GTP酶,将结合在Ras蛋白上的 GTP水解成GDP,成为失活型的 Ras蛋白—GDP。所以在正常情况下,Ras蛋白基本上都与 GDP结合在一起,定位在细胞质膜内表面上。
Ras and Rac as Activators of Reactive Oxygen Species (ROS)
, and have been identified as mediators of a Rac-dependent anti-apoptotic signal in cells microinjected with activated Ras (1 ,2 ). The beneficence of ROS on Ras-transformed cells carries with it the macroscopic potential for selection and growth promotion of cancerous
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