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- DIMA BIOTECH/缔码生物
- 武汉
- PME-F100001
- 2026年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 保质期：
12个月
- 英文名：
Recombinant Feline IL31 protein with C-terminal 6×His tag
- 库存：
In Stock
- 供应商：
缔码生物（武汉）科技有限公司
- 规格：
10μg
| 产品类型 | ECD Proteins |
|---|---|
| 靶点 | IL31 |
| Uniprot ID | XP_011286140.1 |
| 背景 | IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM, Mar 2008] |
| 货期 | In Stock |
| 产品英文名称 | Recombinant Feline IL31 protein with C-terminal 6×His tag |
| 分子结构 | IL31(Ala27-Gln159) 6×His tag |
| 分子量 | The protein has a predicted molecular mass of 16.2 kDa after removal of the signal peptide. The apparent molecular mass of fIL31-His is approximately 15-25 kDa due to glycosylation. |
| 纯度 | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 别名 | Interleukin-31，IL-31 |
| 标签 | C-6×His tag |
| 有无偶联 | Unconjugated |
| 表达宿主 | HEK293 |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 应用范围 | Research use only |
Figure 1. Feline IL31 Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Feline IL31 Protein, His Tag (PME-F100001) can bind Anti-Feline IL31(dovanvetmab biosimilar) mAb (BME100269) in a linear range of 3.20–400 ng/mL.
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文献和实验His 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面： 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螯合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲液的pH、盐
Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
Low (“Low”)Imidazole Buffer 0.5L 100mM Imidizole 3.4g 5% glycerol 25ml 100% glycerol 50mM Tris-HCl (pH 7.9)50ml 0.5M Tris-HCl (pH 7.9) 0.1% Tween-20 0.5M 100% Tween-20 500mM NaCl50ml 5M NaCl dH2O Fill to 0.5L (start w/ 350ml) High (“High
等） 可以完成一般方法很难完成的分离，如变性和非变性蛋白、功能不同的蛋白的分离 属于一种吸附层析，载量高，具有高效的捕获和浓缩作用。 基本步骤 亲和层析作为一种吸附性层析，主要步骤包括平衡、上样、清洗、洗脱和再平衡步骤 （图10)。由于亲和作用需要在特定的条件下结合和洗脱，亲和层析需要使用特定的结合缓冲液和洗脱缓冲液。 图10 亲和层析的基本步骤 适用性 带有标签的融合蛋白纯化，如His标签蛋白、GST标签蛋白等。 抗体及抗体片段的纯化 其他和某些配基有特异性结合作用的分子的纯化
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