- ￥782 - 4299
- DIMA BIOTECH/缔码生物
- 武汉
- PME100697
- 2025年11月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 保质期：
12个月
- 英文名：
Recombinant human NCL protein with N-terminal 6×His tag
- 库存：
In Stock
- 供应商：
缔码生物（武汉）科技有限公司
- 规格：
10μg/50μg/100μg
| 规格： | 10μg | 产品价格： | ￥782.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥2866.0 |
| 规格： | 100μg | 产品价格： | ￥4299.0 |
| 产品类型 | ECD Proteins |
|---|---|
| 靶点 | NCL |
| Uniprot ID | P19338 |
| 别名 | C23;Nsr1 |
| 背景 | Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008] |
| 产品英文名称 | Recombinant human NCL protein with N-terminal 6×His tag |
| 分子结构 | 6×His tag NCL(Val2-Glu710) |
| 分子量 | The protein has a predicted molecular mass of 77.3 kDa after removal of the signal peptide. The apparent molecular mass of His-NCL is approximately 100-130 kDa due to glycosylation. |
| 纯度 | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| 标签 | N-6×His Tag |
| 有无偶联 | Unconjugated |
| 表达宿主 | HEK293 |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 货期 | In Stock |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 应用范围 | Research use only |
Figure 1. Human NCL Protein, His Tag on SDS-PAGE under reducing condition.
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文献和实验Expressed Protein Ligation for Protein Semisynthesis and Engineering
for NCL aside from the rapid isolation of highly pure recombinant proteins. Expressed protein ligation extends the scope of NCL by overcoming the size limitation of target proteins accessible to synthesis. The intein splicing and EPL
.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
mutation db Antithrombin mutation db Asthma and Allergy gene db BIOMDB - Db of mutations causing tetrahydrobiopterin deficiencies BLMbase - Human BLM mutation db (Bloom snydrom) BTKbase - Human BTK mutation db (X-linked
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