- ￥2450
- AntibodySystem
- France
- RHJ56803
- 2025年07月14日
- WB
- Mouse
- Human, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.
- 克隆性：
Monoclonal
- 适应物种：
Human, Rat
- 保质期：
1 year
- 级别：
科研级
- 库存：
99999
- 供应商：
AntibodySystem
- 宿主：
Mouse
- 应用范围：
WB
- 浓度：
1 mg/ml
- 靶点：
Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/MAP1B LC3 A, MAP1LC3A, Microtubule-associated proteins 1A/1B light chain 3A
- 抗体英文名：
Anti-LC3A/B Antibody (R3W51)
- 抗体名：
Anti-LC3A/B Antibody (R3W51)
- 规格：
100 μg
| Clonality | Monoclonal |
| Species reactivity | Human, Rat |
| Host species | Mouse |
| Isotype | IgG2b |
| Conjugation | |
| Tested applications | WB: 1:500-1:1000 |
| Applications | WB |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Target | Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/MAP1B LC3 A, MAP1LC3A, Microtubule-associated proteins 1A/1B light chain 3A, MAP1A/MAP1B light chain 3 A |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | Q9H492, Q9GZQ8 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | R3W51 |
| Note | For research use only. |
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文献和实验ELISA Procedure for Measuring Serum Antibody Titer
of an antibody is shown in Figure 22. The titer was estimated to be 1 in 2400. Figure 22. Antibody titer graph (X = dilution corresponding to Ab titer) Further Methods for Evaluating Anti-peptide Antibodies If the antibodies are to be used as a reagent
Whole mount antibody staining of zebrafish embryos for markers of segmentation
, 10 minutes each. 3. Incubate overnight at 4oC (cold room) in 0.5 ml primary antibody in 0.2% saponin in PBS. Primary antibodies: (A) znp-1 @ 1/2000 (primary motoneurons) (B)F6@ 1/500 (somite boundaries) (C) no antibody (Control)
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