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文献和实验Quantitation of MicroRNAs by Real-Time RT-qPCR
similarity, a number of quantitative RT-qPCR-based miRNA quantitation methods have been introduced since 2004. The most commonly used methods are stem-loop reverse transcription (RT)-based TaqMan� MicroRNA assays and arrays. The high sensitivity
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Invitrogen KNM4500-01 GenElute Mammalian Genomic DNA Miniprep Kit Sigma-Aldrich G1N10
Real‐Time Quantitative PCR Analysis of Mitochondrial DNA Content
, alternate primers for mtDNA 16S rRNA gene and nuclear β‐2‐microglobulin (β2M ) gene (Table 19.7.2 ), 5 µM working concentration diluted in water iTaq SYBR Green SuperMix with ROX (Bio‐Rad
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