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文献和实验Troubleshooting for PCR and multiplex PCR
seaprately using the same PCR program. If one of the primer pairs yields unspecific products, keep the cycling conditions constant and change other parameters as mentioned above (#1 and #2). Mix equimolar amounts of primers and run the multiplex reaction
用ReadyMixTM PCR Reaction Mix进行拟南芥SSLP
用ReadyMix TM PCR Reaction Mix 进行拟南芥SSLP (32 个样品+3 个对照) 一、PCR 采用SIGMA REDTaq® ReadyMixTM PCR Reaction Mix提供的 试剂 (包括20 mM Tris-HCl, pH 8.3, 100 mM KCl, 3 mM MgCl2, 0.002% gelatin, 0.4 M dNTP
Multiplex PCR is a variant of PCR which enabling simultaneous amplification of many targets of interest in one reaction by using more than one pair of primers. Since its first description in 1988 by Chamberlain et al, this method has been applied
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