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200T
产品货号:XF-J5522
产品规格:200T
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文献和实验of the dsRNA before and after RNase III digestion was run on a 15% non-denaturing acrylamide gel along with a 21 bp chemically synthesized siRNA to GAPDH, which served as a size marker. The gel was stained with ethidium bromide and photographed under UV light
(in base pair)s and Quantity (5 µl application per lane) Pst I Bst II Hind III 11,497 8,454 23,130 95 ng DNA 5,077 7,242 9,416 38.8 4,749 6,369 6,557 27 4,507 5,686 4,507 18.5 2,828 4,822 2,322 9.6 2,559 4,324 2,027
Inducing RNAi with siRNA Cocktails Generated by RNase III
for 1 hour at 37°C. 1A. RNase III efficiently digests dsRNA. One microgram of the dsRNA before and after RNase III digestion was run on a 15% non-denaturing acrylamide gel along with a 21 bp chemically synthesized siRNA to GAPDH, which served as a size marker
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