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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
- 克隆性:
Monoclonal
- 适应物种:
Human
- 保质期:
1 year
- 级别:
科研级
- 库存:
99999
- 供应商:
AntibodySystem
- 宿主:
Mouse
- 应用范围:
IF, WB
- 浓度:
1 mg/ml
- 靶点:
ERBB3, HER3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3, Receptor tyrosine-protein kinase erbB-3
- 抗体英文名:
Anti-ERBB3/HER3 Antibody (R3F97)
- 抗体名:
Anti-ERBB3/HER3 Antibody (R3F97)
- 规格:
100 μg

| Clonality | Monoclonal |
| Species reactivity | Human |
| Host species | Mouse |
| Isotype | IgG1 |
| Conjugation | |
| Tested applications | IF: 1:200-1:1000, WB: 1:500-1:2000 |
| Applications | IF, WB |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.05% Sodium Azide. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Target | ERBB3, HER3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3, Receptor tyrosine-protein kinase erbB-3 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P21860 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | R3F97 |
| Note | For research use only. |
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文献和实验,EDTA 缓冲液适用于多数磷酸化酪氨酸特异性抗体,而柠檬酸盐缓冲液适用于其他多数抗体。每天都需制备新鲜的 1X 溶液。抗体:hospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb #2842样本:蜡包埋人类肺部肿瘤抗原修复缓冲液:柠檬酸盐缓冲液(左)或者 EDTA 缓冲液(右)煮沸装置载玻片在所推荐的缓冲液中加热煮沸一段时间后,才可引起抗原修复。该步骤通常在微波炉或高压锅中进行。虽然,一些研究人员也使用水浴锅,但是,因为该步骤中所选择的装置对染色存在积极或消极的影响
) A431 cells. At 48 h after infection, these cells were stained with ten different primary antibodies (5 g/ml) specific for different cell surface markers: anti-CD15 (clone 2F3; BD, PharMingen, San Diego, CA), anti-ERBB-2 (clone SP77; ref. 5), anti-ERBB
)的水解模式相似。新鲜血浆MALDI-TOF MS读数(上方面板)显示除缓激肽和去Arg-缓激肽外,血浆小肽段的水平极低。加入人工合成的C3f(1 pmol/ml血浆),1份立即取出,其他的在15分钟后取出。样品放置于室温。中间的面板显示C末端Arg在几秒钟内被羧肽酶剪切。随后C3f被氨基肽酶活性进一步降解形成与血清中固有梯度相同的序列梯度。 Brad (–R):去掉C末端Arg的缓激肽;R:Arg; RI:Arg-Ile; H:His; T:Thr; I:Ile; K:Lys; S
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