- ￥4000
- 欣润生物
- 江苏
- IK6003
- 2025年12月09日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Chicken uterine smooth muscle cell line
- 库存：
10
- 供应商：
欣润生物
- 肿瘤类型：
不是
- 细胞类型：
永生化细胞
- ATCC Number：
111
- 品系：
鸡
- 组织来源：
子宫
- 相关疾病：
无
- 物种来源：
鸡
- 免疫类型：
正常
- 细胞形态：
梭形
- 是否是肿瘤细胞：
否
- 器官来源：
子宫
- 运输方式：
常温
- 年限：
成年
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化鸡子宫平滑肌细胞简介：
产品描述：鸡子宫是鸡生殖器官的一部分，是动物胎儿或幼体发育生长的场所。子宫肌层比较厚，由成束或成片的平滑肌组成，肌束间以结缔组织分隔。子宫平滑肌具有收缩功能，收缩受激素的调节，其收缩活动有助于精子向输卵管运送、经血排出以及胎儿娩出。子宫平滑肌细胞的分裂增殖还受性腺激素的影响。
产品货号：IK6003
产品类型: 原代细胞建立的永生化
传代能力: 30代左右
产品形态: 梭形
培养基：永生化鸡子宫平滑肌细胞专用完全培养基，产品编号：IK6003-5
支原体：呈阴性
产品培养条件：37℃，5%CO2
发货方式：常温T25方瓶运输
货期：5-7左右货期
Alpha SMA免疫荧光染色鉴定
Isolation of chicken vasa homolog gene and tracing the origin of primordial germ cells
To obtain a reliable molecular probe to trace the origin of germ cell lineages in birds, we isolated a chicken homolog (Cvh) to vasa gene (vas), which plays an essential role in germline formation in Drosophila. We demonstrate the germline-specific expression of CVH protein throughout all stages of development. Immunohistochemical analyses using specific antibody raised against CVH protein indicated that CVH protein was localized in cytoplasm of germ cells ranging from presumptive primordial germ cells (PGCs) in uterine-stage embryos to spermatids and oocytes in adult gonads. During the early cleavages, CVH protein was restrictively localized in the basal portion of the cleavage furrow. About 30 CVH-expressing cells were scattered in the central zone of the area pellucida at stage X, later 45-60 cells were found in the hypoblast layer and subsequently 200-250 positive cells were found anteriorly in the germinal crescent due to morphogenetic movement. Furthermore, in the oocytes, CVH protein was predominantly localized in granulofibrillar structures surrounding the
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
技术资料