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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Bromothymol blue
- 库存:
询盘
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
76-59-5
- 规格:
B100250-5g/B100250-25g/B100250-100g
| 规格: | B100250-5g | 产品价格: | ¥66.9 |
|---|---|---|---|
| 规格: | B100250-25g | 产品价格: | ¥223.9 |
| 规格: | B100250-100g | 产品价格: | ¥591.9 |
英文名:Bromothymol blue
纯度或规格:ACS, Dye content 95%
SPU:B100250
CAS:76-59-5
存储温度:-
运输条件:常规运输
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文献和实验Cyanine Dye Purification Protocol
Slowly add 111.3 mL (80.95 g) HPLC grade triethylamine; the reaction is exothermic; the solution will become cloudy � wait until it clears before adding more) Add dropwise an additional 10-15 mL acetic acid to adjust the pH to 8
Sequencing off Bacterial Genomic DNA with ABI Big Dye Terminators
The following protocol and cycle conditions courtesy of Cheryl Heiner-ABI--------------------------------------------------------------------------------Protocol:Big Dye Terminator Mix* 16μlThermoFidelase** 1μlPrimer (18-24mer)15-30pmolesGenomic DNA
Measurement of Green Fluorescent Protein Expression and DNA Content in Unfixed Cells
Hoechst dye concentration and staining time for different cell types vary as dye up-take depends on cellular metabolic rates; thus, both have to be determined empirically. In general, dye concentrations between 1m g/ml and 10 m g/ml and incubation times
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