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文献和实验Polyclonal Antibody Production 简单快捷制备多抗方法,可用于western、ELISA和免疫/b
Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. -------------------------------------------------------------------------------- Rabbit
were passed through 1-ml nickel affinity columns (B-PER 6×His fusion protein purification kit from Pierce)that had been pre-equilibrated with 7 M GuHCl in TBS (0.15 M NaCl,50 mM Tris.HCl pH 7.5).The columns were washed thrice with 1 ml 7 M GuHCl
Charting Methods for Internal Quality Control for Competition ELISA
. This assay is available in a kit form. Constant evaluation of the use of the kit is part of what is called internal quality control (IQC). Figure 1 shows an overview of the ELISA scheme described in this chapter. The details of the procedure, which involves
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