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文献和实验-His fusions)or 900 ml (MBP fusions)LB medium (Sambrook et al.,1989)containing kanamycin at 50 µg/ml (the plasmid vector confers resistance to this drug).The cultures were shaken vigorously at 37ºC until the optical density at 600 nm reached 0.5; IPTG
Models of Inflammation: Carrageenan Air Pouch
) ELISA kit(s) specific for inflammatory mediators (e.g., TNF‐α, IL‐1β, IL‐6, IL‐10, prostaglandins; Thermo Scientific) Isotonic phosphate‐buffered saline (PBS
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
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